4/4/2024 0 Comments Ez ladder protein western blot![]() Review other protein ladders in the unstained and prestained protein ladder guide. Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands. ![]() Ready-to-use: Supplied in a loading buffer for direct loading on gels.Do not heat, dilute, add reducing agent before loading. The ladder is supplied in gel loading buffer and is ready to use. Lysates can be diluted into several aliquots in a loading buffer and stored frozen at -80 ☌ until ready for use. This usually involves preparing a lysate to extract the protein from cells or tissues. This prestained protein ladder is designed for monitoring protein separation during SDS polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Before running a western blot, we must make our protein of interest accessible to the antibodies. This product was previously called Prism Ultra Protein Ladder (3.5 - 245 kDa). This product is also sold by Expedeon, an Abcam company as RunBlue™ TriColor Prestained Protein Ladder: NXA6050. Someone recently told me that I should add Laemmli buffer to my ladder so that it is loaded at an equal. Fluorescent western blot of STAT1 and phospho-STAT1. When running a western blot, I usually load less ladder (6uL) than any of my samples (20uL). Lanes: Ladder, 1) 10 µg untreated HeLa lysate, 2) 10 µg IFN-treated, 3) 20 µg of untreated, 4) 20 µg IFN-treated. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). After stripping, the blot was re-probed with anti-STAT1 and imaged. Prestained Protein Ladder ab116029 is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa.
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